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Опис документа

Li M., Zhang H. Y., Liang B.
Novel mutations in -tubulin gene in trichoderma harzianum mutants resistant to methyl benzimidazol-2-yl carbamate

Вид документа:  Складова частина документа 
Мова:  Англійська  Обсяг:  С. 499-503 
УДК:  582.28 

Аннотацiя:

Twelve low resistant (LR) mutants of Trichoderma harzianum with the capability of grow fast at 0.8 g/mL methyl benzimidazol-2-yl carbamate (MBC) were obtained using UV mutagenesis. MR and HR mutants which could grow fast at 10 and 100 g/mL MBC, respectively, were isolated by step-up selection protocols in which UV-treated mutants were induced and mycelial sector screening was made in plates with growth medium. Subsequently, -tubulin genes of 14 mutants were cloned to describe the molecular lesion likely to be responsible for MBC resistance. Comparison of the -tubulin sequences of the mutant and sensitive strains of T. harzianum revealed 2 new MBC-binding sites differed from those in other plant pathogens. A single mutation at amino acid 168, having Phe (TTC) instead of Ser (TCC), was demonstrated for the HR mutant; a double mutation in amino acid 13 resulting in the substitution of Gly (GGC) by Val (GTG) was observed in -tubulin gene of MR mutant. On the other hand, no substitutions were identified in the -tubulin gene and its 5-flanking regions in 12 LR mutants of T. harzianum.

Є складовою частиною документа Прикладная биохимия и микробиология. [Текст]. Т. 49. № 5 / РАН. — М. : Наука, 2013.

Теми документа

• УДК/5 Математика та природничi науки/58 Ботанiка/582 Систематика рослин